Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Revvity Health Sciences Inc PhenoVue Fluor 647 - Goat Anti-Rabbit Antibody Cross-Adsorbed
PhenoVue Fluor 647 - Goat Anti-Rabbit Antibody Cross-Adsorbed
Revvity Health Sciences Inc HTRF Human RIG-1 Binding Kit, 100,000 Assay Points
HTRF Human RIG-1 Binding Kit, 100,000 Assay Points
Revvity Health Sciences Inc HTRF Human Total Rb Detection Kit, 50,000 Assay Points
HTRF Human Total Rb Detection Kit, 50,000 Assay Points
Molecular Devices Multi-Mode Validation Plate, Absorbance, Fluorescence, Luminescence
The SpectraTest Multi-Mode Validation Plate is an optical validation package that qualifies absorbance, fluorescence, luminescence, time-resolved fluorescence, and fluorescence polarization performance of compatible multi-mode microplate readers. SoftMax Pro software automates test execution, calculation, and reporting, and flags parameters that fall outside defined limits.
- Validates absorbance, fluorescence, luminescence, time-resolved fluorescence, and fluorescence polarization.
- Automates tests for linearity, precision, accuracy, and dynamic range.
- Includes alignment checks, signal-to-noise and signal-to-background assessments, and filter checks.
- Requires compatible analysis software version 7.0 or higher for automated protocols.
- Recommended annual recertification to maintain traceability and performance.
Revvity Health Sciences Inc HTRF Human Total IκKα Detection Kit, 10,000 Assay Points
HTRF Human Total IκKα Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF Human & Mouse Total BRD4 Detection Kit, Control Lysate
HTRF Human & Mouse Total BRD4 Detection Kit, Control Lysate
Revvity Health Sciences Inc AlphaLISA Human 7-36 amide GLP1 Detection Kit, 100 Assay Points
AlphaLISA Human 7-36 amide GLP1 Detection Kit, 100 Assay Points
Revvity Health Sciences Inc HTRF Human Total CDK7 Detection Kit, 500 Assay Points
HTRF Human Total CDK7 Detection Kit, 500 Assay Points
Molecular Devices Excitation Filter, 485 nm, 20 nm Bandwidth
Excitation filter for fluorescence microplate assays centered at 485 nm with a 20 nm bandwidth. The filter is designed for installation in filter slide holders and is used to selectively excite blue-range fluorophores in fluorescence intensity and ELISA workflows.
- Precise 485 nm center wavelength for blue-excited fluorophores.
- 20 nm bandwidth for selective excitation and reduced background signal.
- Designed for installation in filter slide holders used by multi-mode microplate readers.
- Suitable for fluorescence intensity assays and ELISA workflows.
- Replaceable optical element for routine maintenance and assay optimization.
Molecular Devices Fluorescence Polarization Filter, 700 nm, Excitation
Molecular Devices 700 nm fluorescence polarization filter designed for fluorescence polarization (FP) microplate assays and near-infrared fluorescence detection. It provides excitation and detection at 700 nm for compatible multi-mode microplate readers.
- Provides excitation and detection centered at 700 nm for fluorescence polarization assays.
- Optimized for fluorescence polarization measurements in microplate formats.
- Enables near-infrared fluorescence detection to reduce background and improve signal-to-noise.
- Designed for easy installation in compatible filter holders and cartridges.
- Suitable for use with multi-mode microplate readers that support user-installable filters.
Molecular Devices Filter Lens, 490 nm, Fluorescence, Microplate Reader
The Molecular Devices 490 nm filter lens is an optical filter designed for fluorescence measurements in microplate readers. It transmits light centered at 490 nm to isolate the desired fluorescence band for excitation or emission measurements.
- Center wavelength 490 nm.
- Optical filter lens for fluorescence excitation or emission measurements.
- Designed for use with compatible multi-mode microplate readers.
- Helps reduce background by blocking out-of-band wavelengths.
- Intended as a replacement or spare filter for routine maintenance and assay optimization.
Molecular Devices Absorbance Filter, 350 nm, 10 nm Bandwidth
Molecular Devices 350 nm absorbance filter with 10 nm bandwidth provides narrowband absorbance detection for microplate assays. It is sold as a replaceable filter accessory for compatible absorbance microplate readers and is intended to improve selectivity for wavelength-specific absorbance measurements.
- Center wavelength: 350 nm
- Bandwidth (full width at half maximum): 10 nm
- Filter type: Absorbance
- Intended application: Narrowband absorbance measurements in microplate assays
- Compatibility: Designed for use in compatible absorbance microplate reader filter assemblies
Molecular Devices Fluorescence Polarization Filter, 465 nm, 35 nm, Polarized
Fluorescence polarization filter set designed for microplate fluorescence assays. Centered at 465 nm with a 35 nm bandwidth, the set provides vertically and horizontally polarized filters and includes a holder for installation in compatible microplate readers.
- Designed for fluorescence polarization measurements on microplate readers.
- Center wavelength 465 nm with a 35 nm bandwidth.
- Includes vertically and horizontally polarized filters for polarization assays.
- Supplied as a set of two filters with a single holder for installation.
- Enables selective excitation and improved signal specificity in fluorescence assays.
Molecular Devices Absorbance Filter, 575 nm, 8 nm Bandwidth
This absorbance filter provides narrowband detection at a 575 nm center wavelength with an 8 nm full-width bandwidth for visible-range absorbance assays. It is designed for use in FilterMax F3 and F5 multi-mode microplate readers.
- Provides narrowband absorbance detection at 575 nm.
- 8 nm full-width bandwidth for selective wavelength measurement.
- Designed for use with FilterMax F3 and F5 multi-mode microplate readers.
- Suitable for visible-range absorbance assays and endpoint measurements.